General Topics covered
Key Words Microscopy Training Topics
How does a microscope work and what does it do
What governs getting a good image
The importance of numerical aperature on lateral and axial resolution.
How to Köhler a microscope.
What is the effect of incorrect Köhler illumination
What do the markings on an objective mean?
Aberration – defects of lenses.
Transmitted light illumination
Reflected light illumination (Epi)
Polarised light microscopy
Differential interference contrast (DIC/Normaski)
Setting the camera for the correct brightness, colour and contrast
Saving as different file types (movies etc) and batch file conversion
Scaling, resolutions, repeatability
Light Microscopy Basics
Basic principles and function
Resolution and magnification
Conjugate planes, Köhler illumination
NA and resolution
Ergonomics and maintenance
Differential interference contrast
Circular differential interference contrast
Extending conventional Microscopy
Large area microscopy – stitching
Large area microscopy – panoramic imaging
Optical image stacking (z stack, extended focus)
Topographical images and measurements
Specialist applications non metallic inclusions, heated stages,
Particle measurement. grains, graphite, hardness testing,
Stereo Microscope or Compound Microscope
Growing overlap, which is best for which type of sample
Depth of field considerations compared to compound
Objective working distances
Automation opens new possibilities.
Moving towards complete automation.
Lighting techniques LED, fibre optics, pol bright field darkfield
Using digital cameras on a microscope
Connecting to the microscope
Sensor / chip sizes, field of view, vignetting
Relative camera resolutions
Pixel bit depth & sampling
C mount adapters
Limits of resolution
Magnification and objective resolution
Resolution and its limits
Depth of field
Extending microscope depth of field